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Hint:Teminism is commonly known as reverse transcription, discovered by Temin and Baltimore in 1978.
Complete answer:
Teminism is a theory. It is popularly known as reverse transcription. In molecular biology, Teminism theory explains that RNA can act as a template for the DNA formation, that is DNA can be synthesized from RNA. The reverse transcription begins when the viral particle enters the cytoplasm of a target cell. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized.
The process of reverse transcription generates, in the cytoplasm, a linear DNA duplex with an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA.
In the central dogma, DNA codes for MRNA, which codes for the protein. One known exception to the central dogma is exhibited by retroviruses. These RNA-encoded viruses have a phase in their life cycle in which their genomic RNA is converted back to DNA by a virally encoded reverse transcriptase.
The ability to convert RNA to DNA is a method that is desirable in the laboratory for numerous reasons. Example: Converting RNA of interest to CDNA is used in RT-PCR as well as in other applications like microarray analysis. In the reverse transcriptase PCR, CDNA is constructed from RNA using a reverse transcriptase enzyme to study gene expression.
Note: A reverse transcriptase is an enzyme used to generate complementary DNA (CDNA) from an RNA template, a process termed reverse transcription.
Complete answer:
Teminism is a theory. It is popularly known as reverse transcription. In molecular biology, Teminism theory explains that RNA can act as a template for the DNA formation, that is DNA can be synthesized from RNA. The reverse transcription begins when the viral particle enters the cytoplasm of a target cell. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized.
The process of reverse transcription generates, in the cytoplasm, a linear DNA duplex with an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA.
In the central dogma, DNA codes for MRNA, which codes for the protein. One known exception to the central dogma is exhibited by retroviruses. These RNA-encoded viruses have a phase in their life cycle in which their genomic RNA is converted back to DNA by a virally encoded reverse transcriptase.
The ability to convert RNA to DNA is a method that is desirable in the laboratory for numerous reasons. Example: Converting RNA of interest to CDNA is used in RT-PCR as well as in other applications like microarray analysis. In the reverse transcriptase PCR, CDNA is constructed from RNA using a reverse transcriptase enzyme to study gene expression.
Note: A reverse transcriptase is an enzyme used to generate complementary DNA (CDNA) from an RNA template, a process termed reverse transcription.
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